Protective and Antigenotoxic Potentials of Lantana camara Linn. Leaves L. Roboca1, M. Billacura2 and F. Abamo1 1Biology Dept., 2Chemistry Dept., Mindanao State University, Marawi City, Philippines

L. Roboca, M. Billacura, F. Abamo

Abstract


Peripheral blood micronucleus assay was used to evaluate the antigenotoxic and protective potentials of crude methanolic extract of air-dried leaves of Lantana camara (Linn.) against methyl methanesulfonate (MMS)-induced genotoxicity in albino mice.  Mice pre-treated with MMS prior to extract treatments served as the antigenotoxic tests while those pre-treated with the extract before MMS treatment were the protective tests. The frequencies of micronuclei in the erythrocytes of the treated mice were scored 36 hours after the last treatment. Results demonstrated that the extract has possessed both the protective and antigenotoxic properties against MMS-induced genotoxicity in mice in a dose-dependent manner. The study indicated that the plant could be a potential source of cure against genotoxic-related problems.


Keywords


Keywords: Micronucleus assay, Lantana camara Linn., methyl methanesulfonate, antigenotoxicity test, protective test

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References


CSGMT (The Collaborative Study Group for the Micronucleus Test). (1992). Micronucleus test with mouse peripheral blood erythrocytes by acridine orange supravital staining: The summary report of the 5th collaborative study by CSGMT/JEMS.MMS. Mutation Research, 278, 83-89.

Gentile, N., Mañas, F., Bosch, B., Peralta, L., Gorla, N., Aiassa. D. (2012). Micronucleus assay as a biomarker of genotoxicity in the occupational exposure to agrochemicals in rural workers. Bulletin of Environmental Contamination and Toxicology, 88(6), 816-22.

Goud, V. K., Polasa, K., Krishnaswamy, K. (1993). Effect of turmeric on xenobiotic metabolizing enzymes. Plant Foods for Human Nutrition, 44(1), 87-92.

Heddle, J. A. (1973). A Rapid In Vivo Test for Chromosomal Damage. Mutation Research, 18, 187-190.

IARC (International Agency for Research on Cancer). (1974). Methyl methanesulfonate. In Some Anti-thyroid and Related Substances, Nitrofurans and Industrial Chemicals. IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals to Humans, 7, 253-260.

Joy, J. M., Vamsi, S., Satish, C., Nagaveni, K. (2012). Lantana camara Linn: A REVIEW. International Journal of Phytotherapy, 2(2), 66-73.

Kalita, S., Kumar, G., Karthik, L., Rao, K. V. B. (2012). A Review on Medicinal Properties of Lantana camara Linn. Research Journal of Pharmacy and Technology, 5(6), 711-715.

Kumar, M. S., Maneemegalai, S. (2008). Evaluation of larvicidal effect of Lantana camara Linn. against mosquito species Aedes aegypti and Culex quinquefasciatus. Advances in Biological Research, 2, 39-43.

Merck. (1989). The Merck Index, 11th ed. Rahway, NJ: Merck & Company, Inc.

Oda, H., Nakatsuru, Y., Shimizu, S., Yamazaki, Y., Nikaido, O., Ishikawa, T. (1995). Quantitative detection of ultraviolet light-induced photoproducts in mouse skin by immunohistochemistry. Japan Journal on Cancer Research, 86(11), 1041-8.

Premkumar, A., Kreitman R. J., Kindler, H., Willingham, M. C., Pastan, I. (2004). Phase I study of SS1P, a recombinant anti-mesothelin immunotoxin given as a bolus I.V. infusion to patients with mesothelin-expressing mesothelioma, ovarian, and pancreatic cancers. Clinical Cancer, 13(17), 5144–5149.

Shah, S. U. 2012. Importance of Genotoxicity & S2A guidelines for genotoxicity testing for pharmaceuticals. IOSR Journal of Pharmacy and Biological Sciences, 1(2), 43-54.

Sylianco, C. Y. L. 1998. Philippine Science Encyclopedia: Pharmaceutical and Chemical Sciences, National Research Council of the Philippine.


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